BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF LEISHMANIA PARASITES ISOLATED FROM AN ENDEMIC FOCUS IN EASTERN SUDAN

Authors
OSKAM L PRATLONG F ZIJLSTRA EE KROON CCM DEDET JP KAGER PA SCHONIAN G GHALIB HW ELHASSAN AM MEREDITH SEO
Citation
L. Oskam et al., BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF LEISHMANIA PARASITES ISOLATED FROM AN ENDEMIC FOCUS IN EASTERN SUDAN, Transactions of the Royal Society of Tropical Medicine and Hygiene, 92(1), 1998, pp. 120-122
Citations number
11
Categorie Soggetti
Public, Environmental & Occupation Heath","Tropical Medicine
Journal title
Transactions of the Royal Society of Tropical Medicine and Hygiene → ACNP
ISSN journal
00359203
Volume
92
Issue
1
Year of publication
1998
Pages
120 - 122
Database
ISI
SICI code
0035-9203(1998)92:1<120:BAMCOL>2.0.ZU;2-R
Abstract
Twelve Leishmania isolates from visceral leishmaniasis patients in eas tern Sudan were characterized using isoenzyme analysis, Southern blott ing and polymerase chain reaction (PCR) 'fingerprinting'. Isoenzyme an alysis revealed the presence of 3 zymodemes: MON-18, MON-30 and MON-82 , corresponding to Leishmania donovani sensu stricto, L. infantum and L. archibaldi (still of uncertain taxonomic status), respectively. Sou thern blotting and PCR 'fingerprinting' revealed identical patterns fo r all 3 zymodemes, which were indistinguishable from those of L. donov ani s.s.