The usefulness of overpressured layer chromatography (OPLC) to separat
ion of the multicomponent samples is demonstrated. The instruments use
d, their properties and applications are described. The advantages of
OPLC are confirmed by comparison with TLC and HPLC. Some problems with
presented technique are also indicated. The possibility of solving an
alytical problems by OPLC, e.g. in pharmacy, medicine and industry are
discussed. Special attention is paid to analysis of biologically acti
ve compounds isolated from plant extracts.