Jtc. Tzen et al., COEXISTENCE OF BOTH OLEOSIN ISOFORMS ON THE SURFACE OF SEED OIL BODIES AND THEIR INDIVIDUAL STABILIZATION TO THE ORGANELLES, Journal of Biochemistry, 123(2), 1998, pp. 318-323
The oil bodies of plant seeds contain a triacylglycerol matrix surroun
ded by a monolayer of phospholipids embedded with alkaline proteins te
rmed oleosins, Two distinct oleosin isoforms with molecular masses of
18 and 16 kDa are present in rice oil bodies, Chicken antibodies raise
d against oleosin 18 kDa and rabbit antibodies raised against oleosin
16 kDa did not cross-recognize these two homologous isoforms, This pec
uliar non-cross recognition was used to locate the two oleosin isoform
s on the surface of oil bodies via immuno fluorescence detection using
anti-chicken IgG conjugated with FITC (fluorescein isothiocyanate) an
d anti-rabbit IgG conjugated with Texas-Red, The results revealed that
both oleosin isoforms resided on each oil body in vivo and in vitro.
Artificial oil bodies were reconstituted via sonication using triacylg
lycerol, phospholipid, and oleosins, The results indicated that the tw
o rice oleosin isoforms could stabilize artificial oil bodies individu
ally whereas oleosin 16kDa provided better stability to the organelles
than oleosin 18 kDa.