A. Felinger et G. Guiochon, COMPARING THE OPTIMUM PERFORMANCE OF THE DIFFERENT MODES OF PREPARATIVE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 796(1), 1998, pp. 59-74
Citations number
24
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A comparative study of the optimization of the different modes of the
preparative separation of binary mixtures by liquid chromatography is
presented. Band profiles were calculated by means of the equilibrium-d
ispersive model of chromatography in the cases of isocratic elution, g
radient elution, and displacement chromatography. The objective functi
on to be maximized was the product of the production rate and the reco
very yield. The production rate was calculated using the same definiti
on of the cycle time in all cases. This common definition accounts for
column regeneration after each run in each mode of the separation. Th
e calculations reveal that the number of experimental parameters to be
adjusted to achieve optimum separations is relatively small. The majo
r parameters are the loading factor and the number of theoretical plat
es, besides the displacer concentration in displacement chromatography
, or the gradient steepness in gradient elution. The relative advantag
es of the different modes of preparative chromatography are discussed.
(C) 1998 Elsevier Science B.V.