WATER AS A RESERVOIR FOR CAMPYLOBACTER-JEJUNI INFECTION IN COWS STUDIED BY SEROTYPING AND PULSED-FIELD GEL-ELECTROPHORESIS (PFGE)

The occurrence of campylobacters was studied in the faecal samples of a dairy herd with about 20 animals and in the lake which was their sou rce of drinking water during the grazing period from June to September . Of the total of 141 faecal samples studied, 0-21% were found to be p ositive for C. jejuni at various sampling times throughout the year. M ore cows were found to be campylobacter positive in summer or in autum n after the grazing period than after the winter, when the animals wer e inside and their drinking water source was municipal chlorinated tap water. C. jejuni was isolated from most of the lake water samples. Se rotyping with heat stable antigens and molecular typing with PFGE usin g SacII- and SmaI digested DNA revealed that an animal that was perman ently infected with C. jejuni sero-/PFGE-type PEN 0/6, 25/I/ND most pr obably contaminated the lake water in summer 1987. This was the only s ero/PFGE-type isolated from the lake water in summer and autumn 1987 a nd in spring 1988. This sero/PFGE-type was also isolated from four oth er cows in autumn 1987, suggesting that lake water was the source of t he infection. This study is first to employ molecular methods to asses s the possible role of contaminated drinking water in the transmission of campylobacter infection within a dairy herd.