IMMUNOHISTOCHEMICAL ANALYSIS OF APOCRINE BREAST-LESIONS - CONSISTENT OVER-EXPRESSION OF ANDROGEN RECEPTOR ACCOMPANIED BY THE LOSS OF ESTROGEN AND PROGESTERONE RECEPTORS IN APOCRINE METAPLASIA AND APOCRINE CARCINOMA IN-SITU

Apocrine phenotype is observed in a spectrum of breast epithelial lesi ons spanning from benign metaplasias to apocrine carcinoma. Apocrine m etaplasia is a common finding in fibrocystic change of the female brea st. In situ and invasive apocrine carcinomas are rare variants of duct al carcinoma. All breast apocrine lesions were shown to be associated with increased androgen hormones metabolism. We have evaluated 10 case s of apocrine metaplasia, 3 cases of in situ apocrine carcinoma and 10 cases of invasive apocrine carcinomas using immunostaining method for steroid hormone receptors (estrogen, progesterone, androgen), p53, bc l-2 and BRST-2. Paraffin embedded tissue and avidin-biotin peroxidase complex system were used. Androgen receptor (AR) expression is consist ently increased in all cases of apocrine metaplasia when compared with surrounding normal, non-apocrine breast epithelium. This androgen rec eptor over-expression is accompanied by the loss of immuno-detectable estrogen and progesterone receptor, and also the loss of bcl-2. An ide ntical pattern of immuno-reactivity is seen in in situ apocrine carcin omas, but it is observed with less frequency in invasive apocrine carc inomas, which only infrequently express AR as the only steroid hormone receptor.