D. Chorvatovicova et al., ULTRASONICATION - THE WAY TO ACHIEVE ANTIMUTAGENIC EFFECT OF CARBOXYMETHYL-CHITIN-GLUCAN BY ORAL-ADMINISTRATION, Mutation research. Genetic toxicology and environmental mutagenesis, 412(1), 1998, pp. 83-89
Carboxymethyl-chitin-glucan (CMCG) isolated from Aspergillus niger was
ultrasonicated to decrease its molecular weight. Ultrasonicated CMCG
with molecular weight 0.19 x 10(-5) was administered either intraperit
oneally or orally prior to cyclophosphamide (CP) injection and its eff
ect on the frequency of micronuclei in polychromatic erythrocytes of m
ouse bone marrow was evaluated. Both ways of CMCG administration signi
ficantly decreased the clastogenic effect of CP. The protective effect
of CMCG was concentration dependent, with a higher decrease achieved
by 200 mg/kg than by 100 mg/kg b.wt. Ultrasonic depolymerization of hi
gh molecular CMCG resulted in its anticlastogenic effect against CP no
t only on intraperitoneal, but also on oral administration, achieved b
y decreasing its molecular weight. Ultrasonication proved to be an eff
icient way to obtain molecules of CMCG able to pass through the cell w
alls of the gastrointestinal tract. (C) 1998 Elsevier Science B.V.