ASSIGNMENT OF THE HUMAN PROGRESSION ASSOCIATED PROTEIN (PAP) TO CHROMOSOME 12P12.3

Analysis of mammary carcinoma cell lines MCF-7 and MCF-7ADR by differe ntial display resulted in the identification of a new putative transme mbrane protein, PAP (Progression Associated Protein). Cell lines MCF-7 and MCF-7(ADR) represent a model system for the identification of gen es involved in the clinical progression of human breast cancer. Accord ing to the functions assigned to some members of its family PAP might be involved in cell cycle regulation and cell-cell interactions. Here we describe the chromosomal localization of the PAP gene by the use of fluorescence in situ hybridization (FISH) with two different genomic DNA probes, derived from the 3'-untranslated and the 5'-translated reg ion of the PAP cDNA sequence. The results of the chromosomal localizat ion experiments indicate that the PAP gene is located on human chromos ome 12p12.3.