MELANOMA DIFFERENTIATION-ASSOCIATED GENE-9, MDA-9, IS A HUMAN GAMMA-INTERFERON RESPONSIVE GENE

Citation
Jj. Lin et al., MELANOMA DIFFERENTIATION-ASSOCIATED GENE-9, MDA-9, IS A HUMAN GAMMA-INTERFERON RESPONSIVE GENE, Gene, 207(2), 1998, pp. 105-110
Citations number
27
Categorie Soggetti
Genetics & Heredity
Journal title
GeneACNP
ISSN journal
03781119
Volume
207
Issue
2
Year of publication
1998
Pages
105 - 110
Database
ISI
SICI code
0378-1119(1998)207:2<105:MDGMIA>2.0.ZU;2-7
Abstract
Subtraction hybridization using a cDNA library prepared from temporall y spaced mRNAs from human melanoma cells treated with recombinant huma n fibroblast interferon (IFN-beta) plus mezerein (MEZ) that induces te rminal differentiation (tester cDNA library) and a temporally spaced c DNA library prepared from actively proliferating melanoma cells (drive r cDNA library) produced a Temporally Spaced Subtracted (TSS) cDNA lib rary. This approach resulted in the identification of melanoma differe ntiation associated (mda) genes displaying both enhanced and suppresse d expression during growth inhibition and differentiation. In the pres ent report, we describe a novel cDNA mda-9 that consists of 2084 nucle otides, and encodes a protein of 298 amino acids with a predicted M-r of approx. 33 kDa. Treatment of human SV40-immortalized normal melanom a cells with immune interferon, INF-gamma, induces growth suppression and enhances mda-9 expression without inducing terminal differentiatio n. These results establish that induction of terminal differentiation in human melanoma cells, using the combination of a type I interferon (IFN-beta) + MEZ, can elicit signaling pathways and gene expression ch anges also regulated by type II immune interferon. (C) 1998 Elsevier S cience B.V.