ISOLATION OF PARVALBUMIN ISOTYPES BY PREPARATIVE HPLC TECHNIQUES

Parvalbumins are highly stable Ca2+ binding proteins, present in large quantities in the sarcoplasmic reticulum in the white muscle of most lower vertebrates and fish. The properties of these proteins make them promising antigens for the use as a specific biomarker for fish speci es identification. Parvalbumin isotypes were isolated, on a preparativ e scale level, by use of size exclusion chromatography (SEC) and anion exchange HPLC. The utility of this technique, with regard to maximizi ng purified isotypes, is discussed.