ORGANIC-SOLVENT EXTRACTION ASSOCIATED WITH HPLC IN THE PREPARATION OFHEMORPHINS FROM BOVINE HEMOGLOBIN PEPTIC HYDROLYSATE

Authors
ZHAO QY PIOT JM
Citation
Qy. Zhao et Jm. Piot, ORGANIC-SOLVENT EXTRACTION ASSOCIATED WITH HPLC IN THE PREPARATION OFHEMORPHINS FROM BOVINE HEMOGLOBIN PEPTIC HYDROLYSATE, Preparative biochemistry & biotechnology, 28(1), 1998, pp. 61-78
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Preparative biochemistry & biotechnology → ACNP
ISSN journal
10826068
Volume
28
Issue
1
Year of publication
1998
Pages
61 - 78
Database
ISI
SICI code
1082-6068(1998)28:1<61:OEAWHI>2.0.ZU;2-U
Abstract
Hemorphins extraction by organic solvents from a hemoglobin peptic hyd rolysate was investigated. About thirteen solvents were used and only the aliphatic alcohols displayed selectivity for hemorphins. The resul ting extracted phases, analyzed by SE-HPLC, RP-HPLC, and mass spectrom etry, proved 1-butanol and 2-butanol to be the best extracting solvent s towards hemorphins. The opioid activity test was carried out followi ng each step of extraction and the obtained results were in agreement with a purification.