CLONING AND EXPRESSION OF A CDNA-ENCODING THE LARGE SUBUNIT OF DUCK REPLICATION FACTOR-C

Authors
GUO JT LIU C MASON WS PUGH JC
Citation
Jt. Guo et al., CLONING AND EXPRESSION OF A CDNA-ENCODING THE LARGE SUBUNIT OF DUCK REPLICATION FACTOR-C, Biochimica et biophysica acta, N. Gene structure and expression, 1395(3), 1998, pp. 293-300
Citations number
19
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1395
Issue
3
Year of publication
1998
Pages
293 - 300
Database
ISI
SICI code
0167-4781(1998)1395:3<293:CAEOAC>2.0.ZU;2-G
Abstract
A cDNA encoding an avian homologue of the large subunit of replication factor C (RFC-L) has been cloned from a duck liver cDNA expression li brary prepared in bacteriophage lambda. The full length cDNA encodes a protein with a predicted size of approximately 130 kDa, consistent wi th the size of the polypeptide detected in duck liver. The duck RFC-L amino acid sequence shares 66.4% and 68.4% identity with mouse and hum an RFC-L proteins, respectively. We identified a 4kb RFC-L mRNA expres sed in most duck tissues. (C) 1998 Elsevier Science B.V.