THE INFLUENCE OF DENTIN PERMEABILITY ON CYTOTOXICITY OF 4 DENTIN BONDING SYSTEMS, IN-VITRO

Citation
S. Bouillaguet et al., THE INFLUENCE OF DENTIN PERMEABILITY ON CYTOTOXICITY OF 4 DENTIN BONDING SYSTEMS, IN-VITRO, Journal of oral rehabilitation, 25(1), 1998, pp. 45-51
Citations number
29
Categorie Soggetti
Dentistry,Oral Surgery & Medicine
ISSN journal
0305182X
Volume
25
Issue
1
Year of publication
1998
Pages
45 - 51
Database
ISI
SICI code
0305-182X(1998)25:1<45:TIODPO>2.0.ZU;2-9
Abstract
Dentine adhesives are often placed directly on dentine from which the smear layer has been removed, the thickness of the dentine is minimal and the potential for diffusion of adhesive components into the pulp i s greatest. The permeability of the dentine is one factor that should be critical to whether sufficient diffusion of adhesive components occ urs to cause damage to pulpal cells. Dentine discs were prepared and d ivided into those with low-, medium-, and high-permeability. They were then treated with four different dentine adhesives, after which the p ulpal side of the dentine was placed in contact with 1 mL of cell-cult ure medium. The medium was collected at 24 h intervals for 168 h, and was then placed on monolayers of human pulpal fibroblasts for 24 h. Th e response of the cells was assessed by succinic dehydrogenase activit y (MTT method). The results showed that four dentine adhesive systems released sufficient components to cause suppression of cellular metabo lism through dentine. High-permeability dentine generally allowed more diffusion of these components, but the effect of dentine permeability depended on the material. On the other hand, the time interval betwee n the application of the bonding agent and collection of the eluant wa s consistently important for all materials. Materials were most cytoto xic at early intervals, and were generally less cytotoxic at later int ervals, although there were exceptions and there was persistent (>15%) suppression of cellular metabolism even at late (168 h) intervals. Th e results suggest that application of these materials to dentine, and particularly dentine with high permeability, poses a potential risk to the health of pulpal tissues.