MAPPING THE GENE FOR SEX-LINKED ANEMIA - AN INHERITED DEFECT OF INTESTINAL IRON-ABSORPTION IN THE MOUSE

The sex-linked anemic (sla) mouse carries an anemia that results from an inherited defect of intestinal iron absorption and provides an idea l model with which to investigate this poorly understood yet clinicall y important process, We have precisely mapped the sin locus within the central region of the X chromosome in relation to a panel of microsat ellite markers. Analysis of over 500 progeny from an intraspecific int ercross and a smaller intraspecific backcross segregating sla establis hed, the following locus order in the sin region: DXMit45-sla-(DXMit16 , it96)-DXMit41-DXMit169-DXMit170-DXMit148-(DXMit18, DXMit171)-DXMit84 -DXMit64. The two microsatellites DXMit16 and DXMit96 are located 0.60 +/- 0.35cM from sla and form the telomeric limit of the sba region, T he mapping of the sta locus is an important first seep to identifying the gene itself. (C) 1998 Academic Press.