ZEBRAFISH GENOMIC LIBRARY IN YEAST ARTIFICIAL CHROMOSOMES

We have constructed a zebrafish yeast artificial chromosome (YAC) Libr ary using genomic DNA isolated from the inbred AB zebrafish strain, Th e average insert size is 470 kb, estimated from analysis of 155 random selected YACs. The library consists of 17,000 clones, providing about a 4.7-fold coverage of zebrafish genome. The YAC clones have been arr ayed in individual wells of 96-well microplates and also pooled to per mit rapid polymerase chain reaction screening of the entire library. W e have also found that the YAC ends can be easily rescued and sequence d from pRML1/pRML2-based mini-YAC clones. (C) 1998 Academic Press.