Rapid, on-site HIV testing with same-day results may improve services
and increase the number of clients who learn their serostatus in devel
oping countries. To validate test performance under field conditions a
nd assess the change in the proportion of clients who learn their sero
status, we conducted a field trial using the Capillus HIV-1/HIV-2 assa
y (Cambridge Diagnostics) at the AIDS information Centre counselling a
nd testing sites in Uganda. Compared to the standard 2-EIA testing alg
orithm, the sensitivity of Capillus was 99.6% (95% CI; 98.5%, 99.9%),
the specificity was 98.8% (95% CI; 98.1%, 99.3%), the positive predict
ive value was 96.5% (95% CI; 94.5%, 97.8%), and the negative predictiv
e value was 99.9% (95% CI; 99.5%, 100%). It took less than 5 min to pe
rform a single test, and results were returned to clients in less than
an hour, during which time clients were counselled. This resulted in
a 27% increase in the proportion of clients who learned their serostat
us and received counselling. We conclude that simple, rapid HIV tests
can be performed accurately on-site within the time frame of a clinic
visit, increasing the number of clients who learn their serostatus and
receive pest-test counselling.