A number of ecotypes of plants and algae with increased tolerance to h
eavy metals have been isolated from sites that are polluted with metal
s leading to their application in bioremediation of contaminated ecosy
stems Glutathione is a key component involved in development of resist
ance/tolerance. The present study was focused on identifying the inhib
itory concentrations and effects of copper, nickel and zinc on the gro
wth of Chlorella sp. and recovery of cells from metal toxicity in the
presence of reduced glutathione (GSH). The observations made during th
e study showed that toxicity of copper, nickel and zinc is ameliorated
in the presence of GSH GSH was also observed to indirectly facilitate
the recovery of functional viability (as indicated by chlorophyll con
tent) of the cells.