Fra. Cagampang et al., VARIATION IN THE EXPRESSION OF THE MESSENGER-RNA FOR PROTEIN-KINASE-CISOFORMS IN THE RAT SUPRACHIASMATIC NUCLEI, CAUDATE-PUTAMEN AND CEREBRAL-CORTEX, Molecular brain research, 53(1-2), 1998, pp. 277-284
Using in situ hybridization, we have examined mRNA expression for five
isoforms of protein kinase C (PKC alpha, beta 1, beta 2, gamma and ep
silon) in the rat suprachiasmatic nuclei (SCN) and other central sites
during the 24 h cycle. The signal for each of these isoforms shows a
marked local density within the SCN. In the absence of photic cues, th
ere are changes in the expression of the mRNAs for the four isoforms t
hat are Ca2+-dependent (alpha, beta 1, beta 2 and gamma), but not for
one of the Ca2+-independent PKCs (epsilon). PKC alpha mRNA exhibits a
monophasic rhythm of expression in the SCN with a peak at early subjec
tive night, circadian time (CT) 14. In contrast, the mRNAs for PKC bet
a 1, beta 2 and gamma show a biphasic rhythm in the SCN with peaks at
early subjective day, CT 0, and early subjective night, CT 14. The fou
r Ca2+-dependent isoforms may therefore subserve phase-related functio
ns within the SCN at the onset of subjective night and, in the case of
beta 1, beta 2 and gamma, also at the onset of subjective day. Variat
ion in the mRNAs for PKC beta 1 and gamma (but not for alpha, beta 2 o
r epsilon) is also found in the caudate putamen and in the cingulate a
nd parietal cortex; the biphasic pattern of expression for these mRNAs
is precisely in phase with that observed in the SCN. The beta 1 and g
amma isoforms may therefore contribute to temporally regulated functio
ns at sites outside the SCN. The present observations raise the possib
ility that receptor-mediated regulation of circadian functions is modu
lated or even gated by circadian changes in intracellular components t
hat participate in distinct signal cascades. (C) 1998 Elsevier Science
B.V.