Jc. Erker et al., RAPID DETECTION OF GB-VIRUS-C RNA BY REVERSE TRANSCRIPTION-POLYMERASECHAIN-REACTION (RT-PCR) USING PRIMERS DERIVED FROM THE 5'NONTRANSLATED REGION, Journal of virological methods, 70(1), 1998, pp. 1-5
Citations number
14
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
A simple reverse transcription-polymerase chain reaction (RT-PCR) proc
edure for the detection of GB virus C (GBV-C) RNA in serum or plasma i
s described. Ln this method, total nucleic acid, extracted from a smal
l volume of human plasma, is reverse transcribed using random hexamers
. An aliquot of cDNA is then utilized in PCR employing GBV-C specific
primers designed to highly conserved regions of the 5'nontranslated re
gion (NTR). For additional sensitivity, a second round of nested ampli
fication is performed. Reactions are analyzed on an agarose gel and sa
mples showing an ethidium bromide stained band of the appropriate size
in the first and second amplification, or in the second amplification
only, are designated to be positive. This protocol allows for the rap
id and sensitive detection of GBV-C infection in human plasma or serum
. (C) 1998 Elsevier Science B.V.