ECTOPIC EXPRESSION OF DAN ENHANCES THE RETINOIC ACID-INDUCED NEURONALDIFFERENTIATION IN HUMAN NEUROBLASTOMA CELL-LINES

Citation
Y. Nakamura et al., ECTOPIC EXPRESSION OF DAN ENHANCES THE RETINOIC ACID-INDUCED NEURONALDIFFERENTIATION IN HUMAN NEUROBLASTOMA CELL-LINES, Biochemical and biophysical research communications, 243(3), 1998, pp. 722-726
Citations number
27
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
243
Issue
3
Year of publication
1998
Pages
722 - 726
Database
ISI
SICI code
0006-291X(1998)243:3<722:EEODET>2.0.ZU;2-0
Abstract
Retinoic acid (RA) plays a major role in neuronal cell differentiation . Neuroblastoma cells differentiate in vitro by extending neurites and forming ganglion-like aggregates in response to Rk In the present stu dy, we have examined a biological role(s) of-DAN in the regulation of RA-mediated cellular differentiation in neuroblastoma cells. RTBM1 and SH-SY5Y cells undergo marked morphological changes associated with a remarkable induction of DAN gene expression when exposed to Rk By tran sfecting an expression vector harboring a rat DAN cDNA into SH-SY5Y se lls, we have obtained two independent transfectants which express a la rge amount of DAN. The forced expression of DAN gene enhanced the neur ite extension in the presence of RA, suggesting that DAN gene product might contain some regulatory role(s) in the RA-induced cellular diffe rentiation in neuroblastoma cells. (C) 1998 Academic Press.