A multivariate statistical method (PLS) was used for a physiological c
haracterization of fungi associated with the cheese environment. The c
ombined effects of pH, salt content, oxygen and carbon dioxide levels
on growth and sporulation were studied. Significant factors affecting
growth were salt content, level of carbon dioxide and temperature. Int
eractions between those factors were illustrated and showed that some
fungi were especially sensitive to carbon dioxide levels and salt cont
ent, at low temperature. Sensitivity to the factors often was more pro
nounced in the early growth phase. Results may aid in eliminating unwa
nted fungal growth during cheese production.