QUANTITATIVE-ANALYSIS OF BIOGENIC-AMINES BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY UTILIZING A FIBER OPTIC FLUORESCENCE DETECTOR

Authors
LINARES RM AYALA JH AFONSO AM GONZALEZ V
Citation
Rm. Linares et al., QUANTITATIVE-ANALYSIS OF BIOGENIC-AMINES BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY UTILIZING A FIBER OPTIC FLUORESCENCE DETECTOR, Analytical letters, 31(3), 1998, pp. 475-489
Citations number
21
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
31
Issue
3
Year of publication
1998
Pages
475 - 489
Database
ISI
SICI code
0003-2719(1998)31:3<475:QOBBHT>2.0.ZU;2-E
Abstract
The biogenic amines, putrescine, cadaverine, spermidine and spermine, dansylated in a microwave oven, were separated by HPTLC, with chlorofo rm:triethylamine (2:1) (v:v) as eluent and silica-gel as stationary ph ase. A fibre optic-based fluorescence instrument for in situ scanning was used for quantitative measurements. The compounds were determined over the range 2-85 ng with relative standard deviations between 0.75- 1.39% and detection limits over the range 1.8-3.0 ng. The method was a pplied for the determination of biogenic amines in plant tissue.