P-31 NMR CONFORMATIONAL STUDIES OF NON-PALINDROMIC DNA DUPLEXES RELATED TO HIV-1 ENHANCER

Authors
TISNE C SIMENEL C HANTZ E DELEPIERRE M
Citation
C. Tisne et al., P-31 NMR CONFORMATIONAL STUDIES OF NON-PALINDROMIC DNA DUPLEXES RELATED TO HIV-1 ENHANCER, Journal de chimie physique et de physico-chimie biologique, 95(2), 1998, pp. 412-417
Citations number
22
Categorie Soggetti
Biology,"Chemistry Physical
Journal title
Journal de chimie physique et de physico-chimie biologique → ACNP
Volume
95
Issue
2
Year of publication
1998
Pages
412 - 417
Database
ISI
SICI code
Abstract
Assignment of all P-31 resonances of 16 base-pair DNA duplexes, (5t)d( CTGGGGACTTTCCAGG)(3t) (5t)d(CCTGGAAAGTCCCCAG)(3t), related to the DNA kappa B Site of the HIV-1 LTR together with a mutated sequence (5t)d(C TGCTCACTTTCCAGG)(3t) (5t)d(CCTGGAAAGTGAGCAG)(3t) was determined by 2D heteronuclear inverse NMR spectroscopy (HSQC-TOCSY and heteronuclear C OSY). (3)JH(3')-P coupling constants for most of the phosphates of the mutated oligomer were obtained using heteronuclear selective COSY.