EXPRESSION OF PSYCHROPHILIC GENES IN MESOPHILIC HOSTS - ASSESSMENT OFTHE FOLDING STATE OF A RECOMBINANT ALPHA-AMYLASE

Authors
FELLER G LEBUSSY O GERDAY C
Citation
G. Feller et al., EXPRESSION OF PSYCHROPHILIC GENES IN MESOPHILIC HOSTS - ASSESSMENT OFTHE FOLDING STATE OF A RECOMBINANT ALPHA-AMYLASE, Applied and environmental microbiology, 64(3), 1998, pp. 1163-1165
Citations number
11
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
3
Year of publication
1998
Pages
1163 - 1165
Database
ISI
SICI code
0099-2240(1998)64:3<1163:EOPGIM>2.0.ZU;2-6
Abstract
alpha-Amylase from the antarctic psychrophile Alteromonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-lab ile alpha-amylase folds correctly when overexpressed in Escherichia co li, providing the culture temperature is sufficiently low to avoid irr eversible denaturation. In the described expression system, a compromi se between enzyme stability and E. coli growth rate is reached at 18 d egrees C.