CHEMILUMINESCENT DETERMINATION OF PYRIDOXINE HYDROCHLORIDE IN PHARMACEUTICAL SAMPLES USING FLOW-INJECTION

A chemiluminescent method using flow injection is described for the de termination of pyridoxine hydrochloride. Its detection limit, linearit y and reproducibility were examined. The method is based on the enhanc ing effect of pyridoxine hydrochloride on the chemiluminescence genera ted by the oxidation of luminol with hydrogen peroxide in aqueous pota ssium hydroxide and sodium oxalate. The proposed method is simple and inexpensive. The chemiluminescence intensity is a linear function of p yridoxine hydrochloride concentration over the range 10-250 mu g ml(-1 ) with a detection limit of 6 mu g ml(-1). The applicability of the me thod was demonstrated by the determination of pyridoxine hydrochloride in different tablet formulations and some dietary sources. (C) 1998 E lsevier Science B.V.