A NOVEL G-PROTEIN-COUPLED RECEPTOR WITH HOMOLOGY TO NEUROPEPTIDE AND CHEMOATTRACTANT RECEPTORS EXPRESSED DURING BONE-DEVELOPMENT

A novel G protein-coupled receptor was isolated from a cDNA derived fr om the cell line NH15-CA2 and a cDNA library from adult mouse brain us ing a PCR cloning strategy, The amino acid sequence of the candidate r eceptor DEZ showed homology to neuropeptide and chemoattractant recept ors, Highest overall homology was found with the orphan receptor GPR-1 (65%), the angiotensin II receptor (62%), and the C5a anaphylatoxin r eceptor (60%), Northern blot analysis of dez revealed a predominant 2. 6 kb mRNA species in NH15-CA2 cells, In situ hybridization experiments showed that dez is differentially regulated during development, with a prominent expression in developing osseous and cartilaginous tissue, It was also detectable in the adult parathyroid glands, hinting at a possible function in bone metabolism. (C) 1997 Academic Press.