5 MONOCLONAL-ANTIBODIES AGAINST GLYCOPHORIN-A OF HUMAN ERYTHROCYTE RECOGNIZE GLYCOPROTEIN OF BOVINE ERYTHROCYTE

To study heterophile blood antigens on erythrocytes between human and experimental or domestic animals, we have produced 295 monoclonal anti bodies (MAbs) to human erythrocyte membrane protein, According to the affinity, reactivity, and titre of the MAbs, we selected 40 clones to study the heterophile blood antigens between human and bovine, chicken , guinea pig, horse, rabbit, sheep, and swine, Five MAbs commonly reac ted with human type A, type B, and type O erythrocytes and reacted wit h bovine erythrocytes as well but did not react with erythrocytes from other species, Other MAbs did not react with erythrocytes from all th e tested animals, These five MAbs reacted with the same erythrocyte me mbrane protein, 90 KD glycophorin A (GPA) of human or 200 KD major gly coprotein and other two components of bovine by immunoblotting and GPA competitive inhibition assay, Furthermore, by enzyme treatment and mo nosaccharide competitive inhibition assay, it was confirmed that these five MAbs recognized antigen epitope of glycosylation free amino acid portion but not glycosylation portion of GPA of erythrocyte membrane.