PRODUCTION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY AGAINST MANNOSE-SENSITIVE HEMAGGLUTININ OF VIBRIO-CHOLERAE

Authors
FALERO G RODRIGUEZ BL VALMASEDA T PEREZ ME PEREZ JL FANDO R ROBERT A CAMPOS J SILVA A SIERRA G BENITEZ JA
Citation
G. Falero et al., PRODUCTION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY AGAINST MANNOSE-SENSITIVE HEMAGGLUTININ OF VIBRIO-CHOLERAE, Hybridoma, 17(1), 1998, pp. 63-67
Citations number
22
Categorie Soggetti
Immunology,"Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Hybridoma → ACNP
ISSN journal
0272457X
Volume
17
Issue
1
Year of publication
1998
Pages
63 - 67
Database
ISI
SICI code
0272-457X(1998)17:1<63:PACOAM>2.0.ZU;2-C
Abstract
We have generated murine monoclonal antibodies (MAb) against Vibrio ch olerae mannose-sensitive hemagglutinin (MSHA) using conventional hybri doma procedures, Seven hybridomas were obtained and one characterized. Hybridoma 2F12/F1 secreted an antibody of the IgG(3) type that reacte d with a 17-kDa antigen corresponding to the product of the mshA gene, This MAb inhibited mannose-sensitive agglutination of chicken erythro cytes by EL tor and O139 vibrios, Vibrios expressing MSHA activity inh ibited binding of the antibody secreted by 2F12/F1 to MSHA-coated micr otiter plates.