DETERMINATION OF GENETIC DIVERSITY WITHIN THE GENUS BIFIDOBACTERIUM AND ESTIMATION OF CHROMOSOMAL SIZE

Pulsed-field gel electrophoresis was proven to be an efficient means o f differentiating 25 strains of Bifidobacterium obtained from culture collections. XbaI, SpeI, DraI restriction enzyme profiles indicated ge nomic heterogeneity among strains. When seven human isolates of bifido bacteria were compared using the same methods, two individual banding patterns were obtained. However, despite its discriminatory potential, pulsed-field gel electrophoresis was shown to be of no value in taxon omic identification. Genomic sizes estimated for eight Bifidobacterium strains ranged from 1.5 Mb to 2.1 Mb.