DEVELOPMENT OF A POLYMERASE-CHAIN-REACTION ASSAY FOR MYCOPLASMA-SALIVARIUM BY USING THE NUCLEOTIDE-SEQUENCE WITHIN AMINOPEPTIDASE MY GENE

Authors
YOSHIKAWA T SHIBATA K KAGA M SATO Y OGUCHI H TOTSUKA Y WATANABE T
Citation
T. Yoshikawa et al., DEVELOPMENT OF A POLYMERASE-CHAIN-REACTION ASSAY FOR MYCOPLASMA-SALIVARIUM BY USING THE NUCLEOTIDE-SEQUENCE WITHIN AMINOPEPTIDASE MY GENE, FEMS microbiology letters, 156(2), 1997, pp. 281-286
Citations number
16
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
156
Issue
2
Year of publication
1997
Pages
281 - 286
Database
ISI
SICI code
0378-1097(1997)156:2<281:DOAPAF>2.0.ZU;2-F
Abstract
A polymerase chain reaction assay for a 278-nucleotide DNA fragment wi thin aminopeptidase My gene of Mycoplasma salivarium was developed. Th e assay amplified M. salivarium DNA, but did not amplify DNAs of other mollicutes, bacteria and mammalian cells. The detection limit of the assay was 10 fg of DNA, approximately equivalent to 10 organisms.