LACTATED RINGERS SOLUTION RESUSCITATION CAUSES NEUTROPHIL ACTIVATION AFTER HEMORRHAGIC-SHOCK

Purpose: To determine the degree of neutrophil activation caused by he morrhagic shock and resuscitation. Methods: Awake swine underwent 15-m inute 40% blood volume hemorrhage, and a 1-hour shock period, followed by resuscitation with: group I, lactated Ringer's solution (LR); grou p II, shed blood; and group III, 7.5% hypertonic saline (HTS). Group T V underwent sham hemorrhage and LR infusion. Neutrophil activation was measured in whole blood using flow cytometry to detect intracellular superoxide burst activity. Results: Neutrophil activation increased si gnificantly immediately after hemorrhage, but it was greatest after re suscitation with LR (group I, 273 vs, 102%; p < 0.05). Animals that re ceived shed blood (group II) and HTS (group III) had neutrophil activi ty return to baseline state after resuscitation. Group IV animals had an increase in neutrophil activation (259 vs. 129%; p < 0.05). Conclus ion: Neutrophil activation occurring after LR resuscitation and LR inf usion without hemorrhage, but not after resuscitation with shed blood or HTS, suggests that the neutrophil activation may be caused by LR an d not by reperfusion.