TELOMERASE ACTIVITY IN CERVICAL-CANCER IS QUANTITATIVELY DISTINCT FROM THAT IN ITS PRECURSOR LESIONS

Studies using the telomeric repeat amplification protocol (TRAP) assay have demonstrated telomerase activity not only in cancers but also in non-cancerous lesions. However, quantitative differences in activity between both lesions have not been examined, In the present study, usi ng a stretch PCR assay, telomerase activity was analyzed quantitativel y in cervical cancer, its precursor squamous intra-epithelial lesions (SILs) and normal cervix, In stretch PCR assay, telomerase activity wa s expressed in relative units vs, control activity from C33A cells (10 0 units), Mean telomerase activities in cervical cancer, SIL and norma l cervix were 72 +/- 35 units, 18 +/- 17 units and 7 +/- 4 units, resp ectively, suggesting that telomerase activity in cancer lesions was qu antitatively distinct from that in pre-malignant lesions, which may me an a much more pronounced activation of telomerase in cancers than in SIL. Our findings also suggest that stretch PCR assay can distinguish telomerase activity in cancer from that in non-cancerous lesions and m ay be useful for the differential diagnosis of cancer lesions. (C) 199 8 Wiley-Liss, Inc.