N. Caillol et al., PANCREATIC BILE-SALT-DEPENDENT LIPASE ACTIVITY IN SERUM OF DIABETIC-PATIENTS - IS THERE A RELATIONSHIP WITH GLYCATION, Clinical science, 94(2), 1998, pp. 181-188
1. Pancreatic bile-salt-dependent lipase has been detected in human pl
asma where it has the capability to modify normal low-and high-density
lipoprotein composition and structure and to reduce the atherogenicit
y of oxidized low-density lipoprotein (Shamir R, Johnson WJ, Morlock-F
itzpatrick K, Zolfaghari R, Li L, Mas E, Lombardo D, Morel DW, Fisher
EA, Pancreatic carboxyl ester lipase: a circulating enzyme that modifi
es normal and oxidized lipoproteins in vitro. J Clin Invest 1996; 97:
1696-704). 2. In the present study, we investigated the effect of glyc
ation and particularly that of human serum albumin on the activity of
bile-salt-dependent lipase, lit vitro, bile-salt-dependent lipase acti
vity decreased in the presence of human serum albumin; however this wa
s less pronounced in the presence of glycated human serum albumin. In
vivo, bile-salt-dependent lipase specific activity was about 2-fold hi
gher in the sera of diabetic patients than in the sera of normal subje
cts. 3. A significant increase in the specific activity of bile-salt-d
ependent lipase related to the serum level of glycation was observed.
The increase in bile-salt-dependent lipase specific activity was not r
elated to the glucose concentration in serum suggesting that glycation
of bile-salt-dependent lipase could not be involved in the observed e
ffects. Although the stability of serum bile-salt-dependent lipase was
important enough to allow a systemic action of the enzyme on lipoprot
eins, it could not explain the higher activity of the enzyme in diabet
ic serum. 4. We concluded that bile-salt-dependent lipase could be hel
pful against the premature development of atherosclerosis in diabetes.