The virucidal action of several commercially available disinfectant pr
eparations was assayed against hepatitis A virus and human rotavirus d
ried on polystyrene. Overall, the level of virus disinfection achieved
was very poor, usually inducing less than 3 log titre reduction. Susp
ension tests performed with the same disinfectants showed different vi
rus inactivation rates, thus failing to provide a reliable indication
of the actual virus disinfection on fomites. In our studies, bacteriop
hages of Bacteroides fragilis proved to be a simple, cheap and reliabl
e screening tool for the evaluation of virus disinfection on non-porou
s surfaces. The same conclusion cannot be drawn for poliovirus.