BINDING OF THE 51-KDA AND 42-KDA INDIVIDUAL COMPONENTS FROM THE BACILLUS-SPHAERICUS CRYSTAL TOXIN TO MOSQUITO LARVAL MIDGUT MEMBRANES FROM CULEX AND ANOPHELES SP. (DIPTERA, CULICIDAE)
Jf. Charles et al., BINDING OF THE 51-KDA AND 42-KDA INDIVIDUAL COMPONENTS FROM THE BACILLUS-SPHAERICUS CRYSTAL TOXIN TO MOSQUITO LARVAL MIDGUT MEMBRANES FROM CULEX AND ANOPHELES SP. (DIPTERA, CULICIDAE), FEMS microbiology letters, 156(1), 1997, pp. 153-159
Individual components (P51 and P42) from the crystal toxin (Bin) of Ba
cillus sphaericus were used for in vitro binding competition experimen
ts with brush border membranes (BBMFs) from Culex pipiens and Anophele
s gambiae larval midguts. P51 competed for the Bin binding site with a
similar affinity to the Bin toxin, on both BBMFs. For C. pipiens, P42
bound nonspecifically until P51 was added with maximum binding of P42
at a molar ratio of each component. The binding of P42 was much great
er on A. gambiae BBMFs and the presence of either P51 or P42 enhanced
the binding of the other component, with highest binding when a mole t
o mole ratio of each protein was supplied.