EFFECTIVENESS OF CROSS-LINKED PHYLLOSILICATES FOR INTERCALATIVE IMMOBILIZATION OF SOYBEAN LIPOXYGENASE

A novel procedure was developed to intercalate enzymes into dispersed phyllosilicates that were cross-linked with silicate polymers formed b y the hydrolysis of tetramethyl orthosilicate (TMOS). Lipoxygenase (LO X) intercalated into cross-linked phyllosilicates exhibited high enzym atic activity. The enzyme-phyllosilicate composite prepared by this pr ocedure had an improved pore network. Alkylamines were used to occupy the charge sites of the phyllosilicate, which increased the hydrophobi city of the phyllosilicate and reduced charge-charge interaction betwe en LOX and the phyllosilicate. The amount of macropores and the enzyma tic activity of the Lipoxygenase-phyllosilicate composites increased w ith an increase in the ratio of trimethylammonium (TMA)-phyllosilicate to cross-linking reagent TMOS. LOX intercalatively immobilized into p hyllosilicates displayed good storage stability and reusability at amb ient temperature.