INTERACTION OF THE ADENOVIRUS 14.7-KDA PROTEIN WITH FLICE INHIBITS FAS LIGAND-INDUCED APOPTOSIS

Adenovirus type 5 encodes a 14.7-kDa protein that protects infected ce lls from tumor necrosis factor-induced cytolysis by an unknown mechani sm, In this report, we demonstrate that infection of cells with an ade novirus vector expressing Fas ligand induced rapid apoptosis that was blocked by coinfection with a virus expressing 14.7K. Moreover, AdFasL /G infection resulted in the rapid activation of DEVD-specific caspase s, and caspase activation was blocked by coinfection with Ad14.7/G. Ce ll death induced by the overexpression of Fas ligand, Fas-associated d eath domain-containing protein (FADD)/MORT1, or FADD-like interleukin- 1 beta-converting enzyme (FLICE)/caspase-8 in a virus-free system was efficiently blocked by 14.7K expression. Moreover, we demonstrate that 14.7K interacts with FLICE. These results support the idea that FLICE is a cellular target for the 14.7-kDa protein.