Bd. Lewis et Ep. Spalding, NONSELECTIVE BLOCK BY LA3-TRANSDUCTION( OF ARABIDOPSIS ION CHANNELS INVOLVED IN SIGNAL), The Journal of membrane biology, 162(1), 1998, pp. 81-90
Lanthanide ions such as La3+ are frequently used as blockers to test t
he involvement of calcium channels in plant and animal signal transduc
tion pathways. For example, the large rise in cytoplasmic Ca2+ concent
ration triggered by cold shock in Arabidopsis seedlings is effectively
blocked by 10 mM La3+ and we show here that the simultaneous large me
mbrane depolarization is similarly blocked. However, a pharmacological
tool is only as useful as it is selective and the specificity of La3 for calcium channels was brought into question by our finding that it
also blocked a blue light (BL)-induced depolarization that results fr
om anion channel activation and believed not to involve calcium channe
ls. This unexpected inhibitory effect of La3+ on the BL-induced depola
rization is explained by our finding that 10 mM La3+ directly and comp
letely blocked the BL-activated anion channel when applied to excised
patches. We have investigated the ability of La3+ to block noncalcium
channels in Arabidopsis. In addition to the BL-activated anion channel
, 10 mM La3+ blocked a cation channel and a stretch-activated channel
in patches of plasma membrane excised from hypocotyl cells. In root ce
lls, 10 mM La3+ inhibited the activity of an outward-rectifying potass
ium channel at the whole cell and single-channel level by 47% and 58%,
respectively. We conclude that La3+ is a nonspecific blocker of multi
ple ionic conductances in Arabidopsis and may disrupt signal transduct
ion processes independently of any effect on Ca2+ channels.