We compared the measurement of anti-dsDNA by a commercial ELISA test (
DIASTAT), an in-house ELISA and the Crithidia luciliae assay in cross-
sectional sera samples of 209 systemic lupus erythematosus (SLE) patie
nts and 64 patients with a variety of rheumatological, autoimmune and
non-autoimmune diseases in Hong Kong, The Crithidia assay was found to
be the least sensitive (17%) but most specific (95%) method for detec
tion of a positive result in SLE patients, The DIASTAT assay has a hig
her sensitivity (68%) but lower specificity (80%) than the in-house EL
ISA test (32% sensitivity and 89% specificity). The positive predictiv
e value of the three assays are comparable at 90-92% while DIASTAT had
the highest negative predictive value (44%), There was good linear co
rrelation (r = 0.7) between the two ELISAs. ELISA can serve as a usefu
l screening test for anti-dsDNA in SLE patients and doubtful cases can
then be confirmed by another method such as radio-immunoassay.