ATRIAL-NATRIURETIC-PEPTIDE HAS NO POTENTIAL TO PROTECT AGAINST ENDOTOXIN-INDUCED ACUTE-RENAL-FAILURE IN THE ABSENCE OF RENAL NERVES

Atrial natriuretic peptide (ANP) has been shown to have the potential to restore renal function after ischemic injury, an underlying compone nt of endotoxin (Et)-induced acute renal failure, and is known to coun teract renal sympathetic nerve activity in renal function. We have rec ently found that renal denervation restores the Et-induced renal dysfu nction. The purpose of this study was to examine effects of ANP infusi on on the Et-induced acute renal failure in the absence of renal nerve s. Ten to 14 days after bilateral renal denervation (DNX), Wistar rats (250 to 300 g body wt) were used in the acute experiment. Rats with i ntact renal nerves (INN) served as controls. Following control clearan ce measurements, rats were intravenously injected with 4 mg/kg Et (Esc herichia coli, 055: B5). During endotoxemia, rats were infused with 10 mu g/kg/h ANP or saline vehicle. Et injection reduced the glomerular filtration rate (GFR) significantly in saline-infused INN and DNX rats . ANP infusion restored the greatly reduced GFR to the pre-endotoxemia level in DNX rats but not in INN rats. There was significant differen ce between the ANP-and saline-infused DNX rats in the percentage chang e relative to the basal GFR value during the ANP infusion period. ANP infusion did not improve the hyponatriuresis and oliguria after Et adm inistration, which is independent of renal nerves. In conclusion, ANP infusion has a minor reno-protective effect in rats with Et-induced ac ute renal failure in the absence of the renal nerves.