We describe and validate a computer-video system that records and disp
lays in real-rime rbe activity of multiple specimens in a user-defined
space, at user-defined intervals. The computer program uses image sub
traction algorithms to record changes in video images, and stores obse
rvations in column format or as a series of 2-D matrices. The program
was tested under various lighting conditions, backgrounds, specimen si
ze and specimen speed. An AV model of Macintosh computer with video in
put from a camera or video cassette recorder was used to record and an
alyze the mechanical movement of spots on a turntable and the locomoto
r activity of an ant colony. The limitations and potential application
s of the program are discussed. (C) 1997 Elsevier Science B.V.