ISOLATION OF A DROSOPHILA HOMOLOG OF THE VERTEBRATE HOMEOBOX GENE RX AND ITS POSSIBLE ROLE IN BRAIN AND EYE DEVELOPMENT

Vertebrate and invertebrate eye development require the activity of se veral evolutionarily conserved genes, Among these the Pax-6 genes play a major role in the genetic control of eye development, Mutations in Pax-6 genes affect eye development in humans, mice, and Drosophila, an d misexpression of Pax-6 genes in Drosophila can induce ectopic eyes, Here we report the identification of a paired-like homeobox gene, DRx, which is also conserved from flies to vertebrates, Highly conserved d omains in the Drosophila protein are the octapeptide, the identical ho meodomain, the carboxyl-terminal OAR domain, and a newly identified Rx domain, DRx is expressed in the embryo in the procephalic region and in the clypeolabrum from stage 8 on and later in the brain and the cen tral nervous system, Compared with eyeless, the DRx expression in the embryo starts earlier, similar to the pattern in vertebrates, where Rx expression precedes Pax-6 expression, Because the vertebrate Rx genes have a function during brain and eye development, it was proposed tha t DRx has a similar function, The DRx expression pattern argues for a conserved function at least during brain development, but we could not detect any expression in the embryonic eye primordia or in the larval eye imaginal discs, Therefore DRx could be considered as a homolog of vertebrate Rx genes, The Rx genes might be involved in brain patterni ng processes and specify eye fields in different phyla.