The use of transfected cell lines has provided a powerful approach to
study the functions of transfected proteins, This approach has also pr
oven useful to determine the effects of acute and chronic ethanol expo
sure on particular proteins. We show here, however, that the effects o
f ethanol on transfected proteins vary between transiently transfected
cells and stably transfected cell lines. Moreover, we found that the
effect of ethanol on a particular protein depends on the plasmid vecto
rs used for the transfection.