Si. Aleynik et al., INCREASED CIRCULATING PRODUCTS OF LIPID-PEROXIDATION IN PATIENTS WITHALCOHOLIC LIVER-DISEASE, Alcoholism, clinical and experimental research, 22(1), 1998, pp. 192-196
F-2-isoprostanes (F-2-IP) and 4-hydroxynonenal (4-HNE), peroxidation p
roducts of polyunsaturated fatty acids (PUFA), are considered the most
reliable indicators of endogenous lipid peroxidation in vivo, To dete
rmine to what extent these are also altered in patients with alcoholic
liver disease, plasma free and esterified F-2-IP as well as 4-HNE wer
e measured by GC/MS in 49 fasting subjects who underwent diagnostic pe
rcutaneous needle biopsies of the liver, Compared to patients with mil
d steatosis and no fibrosis, free F-2-IP and 4-HNE were strikingly inc
reased in individuals with alcoholic hepatitis, There was also a signi
ficant but lesser rise of 4-HNE in patients with perivenular fibrosis.
An increase of F-2-IP was also found in subjects with transition to,
or complete, alcoholic cirrhosis, with a comparable trend for 4-HNE, B
y contrast, in patients who were drinking heavily up to 48 hr before a
dmission, F-2-IP were not abnormal, but they increased later (p < 0.00
5), Contrasting with plasma free F-2-IP, esterified F-2-IP were not si
gnificantly changed with fibrosis, Thus, whereas circulating esterifie
d F-2-IP were unchanged in patients with alcoholic liver disease, ther
e was an increase in free F-2-IP as well as 4-HNE during recovery from
intoxication, The increase was not a result of accompanying hepatitis
C but a function of the stage of alcoholic liver injury, possibly ref
lecting enhanced lipid peroxidation as well as interference with bilia
ry excretion and/or hepatic esterification.