Bj. Wards et al., RESTRICTION-ENDONUCLEASE ANALYSIS AND PLASMID PROFILING OF ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPE-7 STRAINS, Veterinary microbiology, 59(2-3), 1998, pp. 175-181
Seventeen serotype 7 Actinobacillus pleuropneumoniae strains isolated
in New Zealand and A. pleuropneumoniae serotypes 1-12 reference strain
s were typed by restriction endonuclease analysis of chromosomal DNA a
nd plasmid profiling. All serotype 7 strains produced similar DNA clea
vage patterns and were significantly different to other reference sero
type strains. Minor differences in the cleavage patterns enabled the 1
7 serotype 7 strains to be grouped into seven profiles. Plasmids were
identified in all but three strains but the banding patterns did not a
ccount for the differences in the chromosomal profiles. The study show
ed that restriction endonuclease analysis and plasmid profiling are us
eful in epidemiological studies of porcine pleuropneumonia. (C) 1998 E
lsevier Science B.V.