CLONING AND CHARACTERIZATION OF THE GENE ENCODING THE IRON-SULFUR PROTEIN OF SUCCINATE-DEHYDROGENASE FROM PLEUROTUS-OSTREATUS

Genomic and cDNA fragments encoding the iron-sulfur protein (Ip) subun it of succinate dehydrogenase (EC 1.3.99.1) have been cloned from the edible basidiomycetous fungus, Pleurotus ostreatus. The gene is interr upted by five introns and is predicted to encode a polypeptide of 268 amino acid residues. Sequence comparison with the Ip subunit from othe r species identified three conserved cysteine-rich clusters. One of th ese contains a critical histidine residue implicated in carboxin sensi tivity in the heterobasidiomycete Ustilago maydis. (C) 1998 Elsevier S cience B.V.