A. Patra et al., SUCCESSFUL PLANT-REGENERATION FROM CALLUS-CULTURES OF CENTELLA-ASIATICA (LINN.) URBAN, Plant growth regulation, 24(1), 1998, pp. 13-16
A successful procedure was established for in vitro plant regeneration
from callus derived from stem and leaf explants of Centella asiatica
on semisolid modified Murashige and Skoog's [7] medium supplemented wi
th 2.0 mg L-1 kinetin and 4.0 mg L-1 a-naphthaleneacetic acid. The rat
e of shoot-bud regeneration was the highest (42.8 and 54.3 shoots/cult
ure in stem and leaf derived callus respectively) after 4 weeks of sub
culture on 4.0 mg L-1 6-benzyladenine, 2.0 mg L-1 Kn, 0.25 mg L-1 a-na
phthaleneacetic acid and 20 mg L-1 adenine sulfate. Differentiated sho
ots rooted within Il days in 1/2 strength MS basal salts supplemented
with 0.5 mg L-1 indole-3-acetic acid and 2% (w/v) sucrose. About 85% o
f rooted plantlets were acclimatized and transferred to the greenhouse
.