SUCCESSFUL PLANT-REGENERATION FROM CALLUS-CULTURES OF CENTELLA-ASIATICA (LINN.) URBAN

A successful procedure was established for in vitro plant regeneration from callus derived from stem and leaf explants of Centella asiatica on semisolid modified Murashige and Skoog's [7] medium supplemented wi th 2.0 mg L-1 kinetin and 4.0 mg L-1 a-naphthaleneacetic acid. The rat e of shoot-bud regeneration was the highest (42.8 and 54.3 shoots/cult ure in stem and leaf derived callus respectively) after 4 weeks of sub culture on 4.0 mg L-1 6-benzyladenine, 2.0 mg L-1 Kn, 0.25 mg L-1 a-na phthaleneacetic acid and 20 mg L-1 adenine sulfate. Differentiated sho ots rooted within Il days in 1/2 strength MS basal salts supplemented with 0.5 mg L-1 indole-3-acetic acid and 2% (w/v) sucrose. About 85% o f rooted plantlets were acclimatized and transferred to the greenhouse .