META-TOPOLIN, A HIGHLY-ACTIVE AROMATIC CYTOKININ FROM POPLAR LEAVES (POPULUS X CANADENSIS MOENCH, CV ROBUSTA)

Authors
STRNAD M HANUS J VANEK T KAMINEK M BALLANTINE JA FUSSELL B HANKE DE
Citation
M. Strnad et al., META-TOPOLIN, A HIGHLY-ACTIVE AROMATIC CYTOKININ FROM POPLAR LEAVES (POPULUS X CANADENSIS MOENCH, CV ROBUSTA), Phytochemistry, 45(2), 1997, pp. 213-218
Citations number
27
Categorie Soggetti
Plant Sciences
Journal title
Phytochemistry → ACNP
ISSN journal
00319422
Volume
45
Issue
2
Year of publication
1997
Pages
213 - 218
Database
ISI
SICI code
0031-9422(1997)45:2<213:MAHACF>2.0.ZU;2-1
Abstract
A rapid analytical screening by high-performance liquid chromatography combined with enzymelinked immunosorbent assay specific for N-6-(meta -hydroxybenzyl)adenine and its 9-substituted derivatives revealed seve ral regions of immunoactivity in extracts of mature poplar leaves (Pop ulusxcanadensis Moench., cv. Robusta). The identity of the cross-react ive compounds was investigated by UV and mass spectrometry and after p ermethylation of unknown substances by gas chromatography-mass spectro metry. Physico-chemical analysis indicated the putative cytokinins are N-6-(meta-hydroxybenzyl)adenine and its 9-beta-D-ribofuranosyl or 9-b eta-D-glucopyranosyl derivatives. The chemical structure was subsequen tly confirmed by synthesis. For N-6(meta-hydroxybenzyl)adenine, a high ly active aromatic cytokinin, we propose the trivial name 'meta-topoli n'. (C) 1997 Elsevier Science Ltd.