MINIATURIZED KINETIC GROWTH-INHIBITION ASSAYS WITH VIBRIO-FISCHERI AND PSEUDOMONAS-PUTIDA (APPLICATION, VALIDATION AND COMPARISON)

Two miniaturized growth inhibition assays with Vibrio fischeri and Pse udomonas putida, based on the German DIN Standards 38 412-37 and 38 41 2-8 are presented. Different calculation modes are compared on the bas is of measuring toxicological effects (EC50 and EC20 values) of severa l heavy metals and other reference substances. They demonstrate the hi gher sensitivity of the Psendomonas putida test. The results point out the insufficiency of the endpoint calculation mode. Therefore, a modi fied integral data analysis procedure is recommended. Possibilities an d limits of the test protocols and kinetic data recordings are discuss ed. (C) 1998 Elsevier Science B.V.