PREPARATION AND APPLICATION OF MACROPOROUS SILICA-BASED POLYMER-BONDED PACKING FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PROTEINS

A novel reversed phase chromatography packing to be used for analytica l protein separations is described. A macroporous silica support was b onded with diethoxymethylvinyl silane, and then copolymerized with hyd roxyethyl methylacrylate and divinylbenzene to produce tailored statio nary phase. This packing has high resolution and stability in extent p H range. It was characterized through the separation of two model prot ein mixtures, the first one containing lysozyme, cytochrome C, beta-la ctoglobulin, chick egg albumin, ribonuclease and pepsin (porcine mucus ), the second mixture containing insulin (two kinds), ribonuclease, ly sozyme, serum bovine albumin. Such manipulations of proteins' elution profile are achievable at run times of less than 15 minutes. II is sug gested that the macroporous silica-based polymer-bonded packing is a b etter alternative for the biopolymer separation.